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KMID : 0379319880130010041
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Korean Journal of Rural Medicine
1988 Volume.13 No. 1 p.41 ~ p.59
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Studies on the Seroimmunodiagnosis of Parasitic Diseases
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林漢鍾/Rim, Han-Jong
李駿商/朱炅煥/嚴基善/鄭明淑/Lee, Joon-Sang/Joo, Kyoung-Hwan/Eom, Kee-Seon/Chung, Myung-Sook
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Abstract
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In many of the helminthic infections, diagnosis is accomplished by the demonstration of the eggs or, occasionally the adult worms or their parts. Diagnosis can be made by the identification of larval stage obtained from stool or surgically extracted materials too. However some kinds of parasitic disease can not be diagnosed by above mentioned procedure alone. Brain cysticercosis, ectopic paragonimiasis, Capillaria hepatica infection in liver is a good example. In such a case, immunologic method would be helpful for the decision of physician.
In this paper, immunologic tools such as indirect hemagglutination test, indirect fluorescent antibody test, circumoval precipitation test, ELISA, western blot were applied for the diagnosis of Clonorchis sinenisis, Cysticercosis and C. hepatica infection and their efficacy was evaluated.
The results obtained were as follows
1) In the diagnosis of clonorchiasis, ELISA revealed sensitiveity of 83.3 %, but cross reeaction against antibody of Paragonimus westermani and Taenia species were observed. Foi the identification of cross reaction and species specific band of Ag-A0. reaction, western blot was applied. 59Kd relative molecular weight and 21Kd band´ were identified as a Clonorchis sinensis specific band.
OD values of ELISA performed with sera of 18 months after praziquantel treatment decreased to half level compared to that of before treatment. Negative conversion rate of ELISA after 18 months of treatment was 60%.
2) In the diagnosis of cysticercosis, IFAT disclosed 95.8 % (23/24) of sensitivity and reaction was most strongly occurred in inner membrane. ELISA revealed 90.0% (36/40) of sensitivity, but cross reaction was observed in both technique. In western blot, 91, 63 and 21Kd Mw bands were identified as a strongly positive band. Among them 63Kd band showed positive reaction against almost all sera of cysticercosis patient.
3) Circumoval precipitation, ELISA, IFAT, showed 85.0% of sensitivity in the diagnosis of C. hepatica infection in rat.
The antigenic localities were inner membrane of sectioned egg antigen on the prectipitates around the mucoid plugs which were induced by circumoval precipitation reaction. Sera from rats infected with 2000eggs were collected periodically to observe the changing patterns of antibody titers by IFAT and ELISA, which showed that high titers were detected at weeks 3 and 5, then gradually declined through weeks 9until to negatively converted at weeks 13.
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